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Stage 7 embryos are in the presomite period and have a well defined embryonic disc with a greatest length between 0.3 and 0.7 mm. The embryonic disc is symmetrical and slightly convex in the plane of its longitudinal axis. The age of stage 7 embryos was initially believed to be about 16 days postfertilization, however, more recently, O'Rahilly and Müller (2001) have reassigned the ages of the Carnegie stages and have placed stage 7 at ca. 18 - 21 days. The stage is characterized by the appearance of the notochordal process and the gastrulation (primitive) node.

The stage is represented in the DREM databases by Carnegie embryo #7802 which has been given a grade of excellent. This specimen has a few of the characteristics of stage 8 embryos and is therefore considered to be an advanced stage 7 embryo. Heuser, Rock & Hertig (1945) estimated the age of this embryo to be 16½ days postovulation. It measures 0.42 mm in length, 0.35 mm in maximum width, and about 0.05 mm in maximum thickness.

This specimen was collected and prepared for microscopic examination in 1940. It was fixed in 70% alcohol and then in Bouin's fluid, embedded in celloidin/paraffin, and serially sectioned transverse to the longitudinal axis at 6 microns. The sections were mounted on glass slides and stained with hematoxylin and eosin. The 100 sections through the embryonic disc and closely adjacent tissue are located on slides 41 to 46. Structures are labeled on every section image.

The morphology of this embryo is well documented in the literature. There are photographs of the implantation site and graphic reconstructions of the specimen. A drawing through the median plane showing the right half of the embryo was made from a model by Mr. J.F. Didusch in 1944. We have generated a digital reconstruction of the median longitudinal section corresponding to this drawing.

The sections have been digitally restored and labeled, and can be viewed at three magnifications. Several 3D reconstructions have been produced from the aligned sections. Labeled and unlabeled animations of the 3D reconstructions together with flythrough animations of the aligned sections are also included on the disk. All of the original section images are available as individual .jpg files or as zip files for anyone who wishes to use them for other reconstructions, research or presentations, .

Instructions for using the disks can be found by going to the Instructions section from the opening screen.

 

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